News / New publications

Research

We work together for a better translational approach, from basic to clinical science

  • Read more

Core facilities

Our advanced core facilities offer excellent opportunities

  • Read more

Recruitment

We welcome the best research talents from Norway and the rest of the world

  • Read more

Archives IEMR

  • Publications
  • News archive
  • Visual profile

About IEMR

  • About
  • Funding
  • Collaborations
  • Staff

Contact IEMR

  • Contact
  • Group leaders
  • Leadership
  • Safety representative (VO)

Tubulator: automated analysis of cardiomyocyte organelles and proteins in 3D

Frisk M, Norseng PA, Stenersen Espe EK, Louch WE

Tubulator: an automated approach to analysis of t-tubule and dyadic organization in cardiomyocytes

—

 

During cardiac disease, intracellular cardiomyocyte organelles and proteins are remodeled and disrupted, thereby reducing cardiac performance. Robust and versatile tools for characterization of such sub-cellular structures are needed.

While analysis programs for continuous and regular structures such as rodent ventricular t-tubules are available, at least in 2D, these approaches are less appropriate for assessment of more irregular structures, such as dyadic proteins and non-rodent t-tubules. In the present paper, a versatile, easy-to-use software that performs such analyses is demonstrated. The software, called Tubulator, enables automated analysis of both tissue sections and isolated myocytes. Importantly, Tubulator provides tools for 3D assessment and rendering of image stacks, extending examinations from the single plane to the whole-myocyte level. This freely accessible program provides detailed automated analysis of the three-dimensional nature of dyadic and t-tubular structures.

Tubulator’a versatility enables advances analysis of tissue sections in 3D. Tubulator is able to perform detailed analysis on mltiple cells from the same section in three dimensions. The image depicts intracellular distances from the cellular membrane and t-tubules through a sequence of images.

Philos Trans R Soc Lond B Biol Sci, 377 (1864), 20210468
PubMed 36189810 DOI 10.1098/rstb.2021.0468

Michael Frisk

Postdoctoral fellow

  • View profile

William (Bill) Louch

Group Leader & Professor

  • View profile

Institute for Experimental Medical Research

Oslo University Hospital, Ullevål

PB 4956 Nydalen

NO-0424 Oslo

Norway

  • Research
  • Core facilities
  • Recruitment
  • Publications
  • News archive
  • Visual profile
  • About
  • Funding
  • Collaborations
  • Staff
  • Contact
  • Group leaders
  • Leadership
  • Safety representative (VO)