Core Facility Super-resolution and Confocal Microscopy

The Core Facility for Advanced Light Microscopy, Ullevål is located at the Institute for Experimental Medical Research, 4th floor, building 7, Oslo University Hospital Ullevål.

To See the Unseen
Core Facility Super-resolution and Confocal Microscopy, IEMR

The Core Facility Microscopy, Ullevål, holds two super-resolution microscopes, two confocal microscopes and three whole cell fluorescence setups as well as software for image enhancement.

Our institute is one of the nodes in a core facility for super-resolution and confocal microscopy financed by Helse Sør-Øst. The two other nodes are located at Montebello and Gaustad.

Booking information

 

Our Equipment:

Zeiss ELYRA LSM 710 / Axio Observer

The Elyra combines three dimensional (PRILM Technology), dual channel dSTORM/PALM super-resolution imaging with standard confocal microscopy to study protein localization and geometry at the nanoscale level. The setup is able to achieve an experimental resolution of 30 nm laterally, and 70 nm axially. The microscope is also suitable for live imaging of fluorescent-tagged transgenic cell lines.

Super-resolution laser lines: 405-488-542-643 nm.
Confocal laser lines: 458-488-514-543-633 nm.

Zeiss LSM 800 Airyscan / Axio Observer

Inverted super-resolution confocal microscope used for studying protein localization, motility and colocalization using traditional antibody labelling. Additionally, the microscope is fitted with an incubation chamber for live-cell imaging experiments requiring control of temperature and O2/CO2 levels. The LSM 800 enables simultaneous imaging of up to 4 different proteins. With Airyscan processing, the setup is able to achieve a resolution of 120 nm laterally and 350 nm axially, far exceeds that of a conventional confocal microscope.

Laser lines: 405-488-561-640 nm.

Zeiss 7 Live / Axio Observer

Inverted confocal microscope used for studying Ca2+ dynamics in live cells. The 7 Live provides high speed scanning and gives acquisition rates of 1000 images of 512 x 512 pixels in less than 10 seconds. The microscope is set up with a perfusion system (37°C), and field stimulation. Flash photolysis equipment is also available.

Laser lines: 488-532 nm.

Zeiss Observer D1 / Cell Tester

This fluorescence system enables wide-field fluorescence detection such as Fluo-4 (Easy Ratio Pro) by high-speed camera (Orca Flash 4.0, Hamamatsu Photonics) with WarpDrive interface controller. The setup is also equipped with a Cell Tester system (World Precision Instruments), enabling concomitant measurements of local cellular Ca2+ dynamics as well as contractile force development at varying sarcomere lengths. Additionally, the setup contains a heated perfusion system, as well as equipment for electrophysiology (Axon Axoclamp 200B) and field stimulation.

Zeiss LSM 510 / Axiovert 100

This inverted point-scanning confocal microscope has modest spatial and temporal resolution, and is commonly employed for both imaging of ionic fluorophores and static imaging protein localization / colocalization. This setup is also equipped with whole-cell fluorescence with PMT-based detection (Ratio Master, Photon Technology International), a heated perfusion system, electrophysiology (Axon Axoclamp 2B).

Laser lines: 488-543-633 nm.

Zeiss Axiovert 200 / Cairn Optoscan
This inverted wide-field microscope is employed for studying ionic dynamics in live cells. Whole-cell fluorescence can be measured using a fluorescence system from Cairn Research (Optoscource / Optoscan, 1 detection channel). The setup is equipped with a heated perfusion system, as well as equipment for measuring cell shortening (Crescent electronics) and electrophysiology (Axon Axoclamp 2B) and for exciting cells by field stimulation.

Zeiss Axio Observer / PTI Wide-Field Fluorescence
This inverted wide-field microscope is employed for studying ionic dynamics in live cells. Whole-cell ratiometric fluorescence (for example Fluo-4, Fura-2, SBFI) can be measured using the Ratio Master system from Photon Technology International employing Felix software. The setup is equipped with a heated perfusion system, equipment for measuring cell shortening (Crescent electronics) and electrophysiology (Axon Axoclamp 2A) and for exciting cells by field stimulation.

Huygens deconvolution software from Scientific Volume Imaging
The core facility is licensed for use of deconvolution software from SVI which improves image quality by reversing optical distortion in the image.

Zeiss LSM 800 Airyscan in use